rabbit anti il 1 β  (Danaher Inc)

Journal: Cancers

Article Title: Study of Biological Effects Induced in Solid Tumors by Shortened-Duration Thermal Ablation Using High-Intensity Focused Ultrasound

doi: 10.3390/cancers16162846

Figure Lengend Snippet: Immunolabeling for IL-1α (red, ( left ) panel) and IL-1β (green, ( right ) panel) in a HIFU-treated tumor (3 days post-sonication) as compared to an untreated control tumor. White arrows indicate immunopositive cells. Bisbenzimide was used for visualization of cell nuclei (blue). Histograms show the intensity of immunolabeling for IL-α and IL1-β per microscopic field of view. The intensity of immunoreactivity is presented as the height of the surface plot profiles.

Article Snippet: Then, the sections were incubated for 1 h at 37 °C with PBS+T containing 1% normal goat serum and one or two of the following primary antibodies (Abs): (1) murine monoclonal Ab against vascular endothelial growth factor (VEGF) (Santa Cruz Biotechnology, Santa Cruz, CA, USA, dil. 1:400); (2) rabbit polyclonal Ab against IL 1-β (Invitrogen-Molecular Probes, Eugene, OR, USA, dil. 1:400); (3) murine monoclonal Ab against IL 1-α (Santa Cruz Biotechnology, CA, USA, dil. 1:400); (4) murine monoclonal Ab against TNF-α (Santa Cruz Biotechnology, CA, USA, dil. 1:400); (5) murine monoclonal Ab against macrophages (Santa Cruz Biotechnology, CA, USA, dil. 1:400); (6) murine monoclonal Ab against lymphocytes CD8 (DAKO, Santa Clara, CA, USA, dil. 1:400), and (7) murine monoclonal Ab against INF-γ (Santa Cruz Biotechnology, CA, USA, dil. 1:400).

Techniques: Immunolabeling, Sonication, Control